Biotin-PEG2-methyl ethanethioate Catalog number: BADC-01512

Biotin-PEG2-methyl ethanethioate, a versatile bioconjugation reagent, finds widespread use in various bioscience applications. Here are four key applications of this compound with a high degree of perplexity and burstiness:

Protein Labeling: Employed extensively in scientific research, Biotin-PEG2-methyl ethanethioate serves as a crucial tool for labeling proteins with biotin, thereby facilitating their detection, purification, and immobilization. Through the biotinylation process, proteins can be efficiently captured on streptavidin-coated surfaces, enabling focused and specific protein interactions. This methodology is routinely utilized in a range of assays, including ELISA tests, Western blotting procedures, and protein tracking experiments.

Drug Delivery Systems: Revolutionizing the field of drug delivery, Biotin-PEG2-methyl ethanethioate enhances targeting precision and delivery efficacy within biological systems. By incorporating this compound into drug delivery systems and biotinylating therapeutic agents, the selective capturing of these agents by biotin-binding proteins is made possible, directing them to specific cells or tissues with precision. This targeted approach minimizes off-target effects and optimizes the therapeutic index of drugs.

Bioconjugate Development: Essential for the creation of bioconjugates, Biotin-PEG2-methyl ethanethioate plays a pivotal role in attaching various biological molecules, such as antibodies, enzymes, or DNA, to surfaces or other entities. These bioconjugates find utility in a variety of applications, from diagnostic assays to biosensors and therapeutic interventions. The inclusion of the PEG2 linker in Biotin-PEG2-methyl ethanethioate adds flexibility and reduces steric hindrance, ultimately enhancing the functional performance of bioconjugates and expanding the possibilities of bioconjugate-based technologies.

Immunoprecipitation: Widely used in molecular biology and biochemistry research, Biotin-PEG2-methyl ethanethioate is instrumental in immunoprecipitation experiments, where it is utilized to biotinylate antibodies or antigens. By biotinylating these molecules, they can be efficiently extracted using streptavidin-coated beads, enabling the isolation and analysis of protein complexes. This technique is indispensable for studying intricate protein-protein interactions, unraveling signal transduction pathways, and investigating post-translational modifications, providing invaluable insights into cellular processes and molecular mechanisms.