DBCO-NHS ester - CAS 1353016-71-3 Catalog number: BADC-00933

DBCO-NHS ester, a highly reactive compound utilized for bioconjugation in biosciences, offers a multitude of applications. Here are four key applications:

Bioconjugation: Widely embraced for its proficiency in conjugating proteins, peptides, and other biomolecules, DBCO-NHS ester forms amide bonds with primary amines on the target molecule, facilitating the attachment of diverse tags, dyes, or functional groups. This process plays a pivotal role in advancing diagnostic assays, drug delivery systems, and therapeutic proteins, serving as a cornerstone for cutting-edge biomedical innovations.

Surface Functionalization: Embracing the versatility of DBCO-NHS ester, researchers can modify the surfaces of nanoparticles, hydrogels, and various materials for a spectrum of biomedical applications. By attaching specific ligands or biomolecules, they elevate the targeting precision, biocompatibility, and functionality of these materials, catalyzing the creation of precise drug delivery systems and biosensors tailored for intricate medical scenarios.

Click Chemistry: At the heart of dynamic biological exploration lies DBCO-NHS ester, a vital component in strain-promoted alkyne-azide cycloaddition (SPAAC), an intricate bioorthogonal “click” reaction. This precise mechanism enables the specific and efficient labeling of biomolecules without disrupting native biological processes, granting scientists unparalleled insight into the dynamic inner workings of live cells and organisms through sophisticated imaging, tracking, and study methodologies.

Immunoassays: In the realm of immunoassays, DBCO-NHS ester takes center stage, facilitating the covalent linkage of antibodies to various labeled probes, like fluorescent dyes or enzymes. This robust conjugation technique enables the detection and quantification of specific antigens within complex samples, heightening assay sensitivity and accuracy.