DBCO-NHS ester 3 - CAS 1393350-27-0

DBCO-NHS ester 3 - CAS 1393350-27-0 Catalog number: BADC-00591

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Category
ADCs Linker
Product Name
DBCO-NHS ester 3
CAS
1393350-27-0
Catalog Number
BADC-00591
Molecular Formula
C24H20N2O5
Molecular Weight
416.43
DBCO-NHS ester 3

Ordering Information

Catalog Number Size Price Quantity
BADC-00591 -- $--
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Canonical SMILES
O=C(CC1)N(OC(CCCC(N2C3=CC=CC=C3C#CC(C=CC=C4)=C4C2)=O)=O)C1=O
InChI
InChI=1S/C24H20N2O5/c27-21(10-5-11-24(30)31-26-22(28)14-15-23(26)29)25-16-19-8-2-1-6-17(19)12-13-18-7-3-4-9-20(18)25/h1-4,6-9H,5,10-11,14-16H2
InChIKey
CFQHVJJWCDJMLI-UHFFFAOYSA-N
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Room temperature

DBCO-NHS ester 3, a click chemistry reagent widely embraced in biosciences, presents diverse avenues for biomolecule conjugation. Here are four key applications:

Bioconjugation: Harnessing the power of DBCO-NHS ester 3 in bioconjugation techniques links proteins, peptides, or other biomolecules with azide-functionalized counterparts, ushering in the era of antibody-drug conjugates, nanoparticles, and biosensors. These conjugates serve as linchpins in targeted drug delivery, diagnostic assays, and therapeutics, signaling a paradigm shift in the realm of precision medicine.

Fluorescent Labeling: Serving as a cornerstone in fluorescence imaging and flow cytometry, DBCO-NHS ester 3 assumes a pivotal role in labeling proteins and biomolecules with fluorophores. By binding fluorescent dyes to specific targets, researchers gain unparalleled insights into cellular processes and molecular interactions in real-time, illuminating cell biology, protein localization, and interaction dynamics with unrivaled clarity.

Surface Functionalization: The versatility of DBCO-NHS ester 3 extends to surface modification of nanoparticles, microarrays, and biosensors with bioactive molecules. This critical functionalization of surfaces creates interfaces capable of selectively binding to biomolecular targets, bolstering the sensitivity and specificity of biosensors and diagnostic tools. This process stands as a linchpin in tissue engineering applications, where surface interactions dictate the outcomes desired in the field.

Drug Delivery Systems: Positioned at the forefront of innovation in drug delivery, DBCO-NHS ester 3 propels the development of novel systems by facilitating the attachment of therapeutic agents to polymers or nanoparticles. This strategic attachment ensures the targeted and controlled release of drugs at precise sites, minimally impacting side effects while maximizing treatment efficacy. The pivotal role of DBCO-NHS ester 3 in advancing precision medicine and nanomedicine technologies heralds a new epoch in personalized healthcare.

1.Chemoenzymatic synthesis of glycoengineered IgG antibodies and glycosite-specific antibody-drug conjugates
Tang F, Wang LX, Huang W
Glycoengineered therapeutic antibodies and glycosite-specific antibody-drug conjugates (gsADCs) have generated great interest among researchers because of their therapeutic potential. Endoglycosidase-catalyzed in vitro glycoengineering technology is a powerful tool for IgG Fc (fragment cystallizable) N-glycosylation remodeling. In this protocol, native heterogeneously glycosylated IgG N-glycans are first deglycosylated with a wild-type endoglycosidase. Next, a homogeneous N-glycan substrate, presynthesized as described here, is attached to the remaining N-acetylglucosamine (GlcNAc) of IgG, using a mutant endoglycosidase (also called endoglycosynthase) that lacks hydrolytic activity but possesses transglycosylation activity for glycoengineering. Compared with in vivo glycoengineering technologies and the glycosyltransferase-enabled in vitro engineering method, the current approach is robust and features quantitative yield, homogeneous glycoforms of produced antibodies and ADCs, compatibility with diverse natural and non-natural glycan structures, convenient exploitation of native IgG as the starting material, and a well-defined conjugation site for antibody modifications. Potential applications of this method cover a broad scope of antibody-related research, including the development of novel glycoengineered therapeutic antibodies with enhanced efficacy, site-specific antibody-drug conjugation, and site-specific modification of antibodies for fluorescent labeling, PEGylation, protein cross-linking, immunoliposome formation, and so on, without loss of antigen-binding affinity. It takes 5-8 d to prepare the natural or modified N-glycan substrates, 3-4 d to engineer the IgG N-glycosylation, and 2-5 d to synthesize the small-molecule toxins and prepare the gsADCs.
2.Copper-free click chemistry on polymersomes: Pre vs. Post-self-assembly functionalisation
Silvie A. Meeuwissen, et al.
The optimal accessibility of functional groups on polymeric nanosized vesicles was investigated with copper-free clickable probes as a model system. Cu-free clickable polymersomes were developed either through co-assembly of end group modified amphiphilic block copolymers or by introduction of the reactive moieties on preformed vesicles. For the co-assembly approach, the highest degree of availability was obtained for the most hydrophilic functional group, whereas hydrophobic species were unable to react as efficiently since they were seemingly buried in the membrane. Post-self-assembly introduction led to good results for all three examined moieties whereby surface saturation was reached above a certain percentage of immobilised probes. Finally, we demonstrated that protrusion of functional entities from the membrane corona via a longer hydrophilic segment of the block-copolymer significantly enhances the accessibility.
The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Historical Records: DBCO-NHS ester 3
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