BS3 Crosslinker - CAS 82436-77-9

BS3 Crosslinker - CAS 82436-77-9 Catalog number: BADC-01165

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BS3 Crosslinker is a non-cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs).

Category
ADCs Linker
Product Name
BS3 Crosslinker
CAS
82436-77-9
Catalog Number
BADC-01165
Molecular Formula
C16H20N2O14S2
Molecular Weight
528.47
BS3 Crosslinker

Ordering Information

Catalog Number Size Price Quantity
BADC-01165 -- $--
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Description
BS3 Crosslinker is a non-cleavable ADC linker used in the synthesis of antibody-drug conjugates (ADCs).
Synonyms
Bis(sulfosuccinimidyl)suberate
IUPAC Name
1-[8-(2,5-dioxo-3-sulfopyrrolidin-1-yl)oxy-8-oxooctanoyl]oxy-2,5-dioxopyrrolidine-3-sulfonic acid
Canonical SMILES
C1C(C(=O)N(C1=O)OC(=O)CCCCCCC(=O)ON2C(=O)CC(C2=O)S(=O)(=O)O)S(=O)(=O)O
InChI
InChI=1S/C16H20N2O14S2/c19-11-7-9(33(25,26)27)15(23)17(11)31-13(21)5-3-1-2-4-6-14(22)32-18-12(20)8-10(16(18)24)34(28,29)30/h9-10H,1-8H2,(H,25,26,27)(H,28,29,30)
InChIKey
VYLDEYYOISNGST-UHFFFAOYSA-N
Solubility
10 mm in DMSO
Melting Point
>300°C
LogP
-0.03120
PSA
255.69000
In Vitro
Treatment of intact cells with the homobifunctional crosslinker BS3 Crosslinker produces similar amounts of covalent dimer in both normal and SAO cells. In SAO cells, copies of normal band 3 can be distinguished from SAO band 3 by treating with H2DIDS to form a crosslink between major chymotryptic fragments (60 kDa and 35 kDa) within one subunit. Successive treatment of cells with [3H]-4,4'-diisothiocyanatodihydrostilbene-2,2'-disulfonate ([3H]H2DIDS), BS3, and chymotrypsin gives 3H-labeled products that include homodimer of normal band 3 as well as products of crosslinking normal band 3 with the 60- and 35-kDa fragment of SAO band 3.
Appearance
Light tan powder
Purity
≥ 97.0 %
Shipping
Room temperature
Storage
Store at -5 °C, keep in dry and avoid sunlight.
Pictograms
Irritant
Signal Word
Warning

BS3 Crosslinker, a bis(sulfosuccinimidyl) suberate crosslinking reagent, is utilized widely in biochemical research for its non-cleavable and homobifunctional properties. Here are four key applications of BS3 Crosslinker:

Protein-Protein Interaction Studies: In the realm of understanding protein interactions, BS3 Crosslinker plays a pivotal role. By covalently linking interacting proteins together, researchers can stabilize transient complexes, enabling detailed analysis. This innovative technique allows for the detection of interaction partners within intricate biological mixtures, shedding light on complex protein networks.

Structural Biology: Delving into the architecture of proteins within complexes, BS3 Crosslinker is a valuable tool in structural biology. Preceding structural characterization techniques like mass spectrometry or cryo-EM, crosslinking proteins with BS3 provides essential distance constraints for precise 3D modeling. This detailed spatial information enhances our grasp of protein structure and function, offering insights into molecular mechanisms.

Cell Surface Receptor Mapping: With its unique water solubility, BS3 Crosslinker aids in mapping cell surface receptors and their interactions. Capable of permeating the cell membrane without infiltrating intracellular components, this reagent facilitates the crosslinking of surface proteins for in-depth study. By dissecting receptor complexes and signaling pathways crucial for cellular communication, BS3 Crosslinker contributes to unraveling the intricacies of cell signaling.

Antibody-Antigen Complex Analysis: In the realm of immunological studies, BS3 Crosslinker emerges as a critical tool for stabilizing antibody-antigen complexes. By crosslinking these complexes, researchers can analyze binding affinities, map epitopes, and investigate receptor-ligand interactions. This in-depth analysis is instrumental in the development of diagnostic tools and therapeutic antibodies, advancing the field of immunotherapy and precision medicine.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Historical Records: BS3 Crosslinker
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