Catalog Number | Size | Price | Quantity | ||
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BADC-01163 | -- | $-- | Inquiry |
DTSSP, or 3,3’-dithiobis(sulfosuccinimidyl propionate), is a widely used water-soluble crosslinker that features amine-reactive NHS-ester groups on both ends of an 8-atom spacer arm. The central disulfide bond in this spacer can be cleaved using reducing agents, making DTSSP particularly versatile in biochemical applications. The sulfo-NHS esters of DTSSP react specifically with primary amines, found commonly in the side chains of lysine residues or the N-terminus of protein polypeptides. This reactivity allows DTSSP to form stable amide bonds, providing a reliable means to link proteins, including antibodies. The cleavable nature of DTSSP’s spacer arm offers a key advantage for applications that require the eventual separation of crosslinked products, setting it apart from non-cleavable crosslinkers like its non-sulfonated analog, DSS.
In drug discovery, DTSSP’s capabilities extend to the study of protein interactions and structural biology. By crosslinking primary amines on protein surfaces, researchers can stabilize weak and transient interactions that might otherwise escape detection. This stabilization allows for the identification and characterization of such interactions, which are crucial for understanding biological pathways and mechanisms of action for potential therapeutics. Additionally, DTSSP is instrumental in immobilizing proteins onto amine-coated surfaces, thus facilitating the development of assays that can screen for drug activity or detect biomarkers. Its success in forming bioconjugates is due largely to its efficiency in creating single-step chemical crosslinks, an important factor in high-throughput screening environments.
The water solubility of DTSSP ensures that it is particularly useful for applications involving cell surface labeling. Its membrane-impermeable nature means it primarily targets extracellular proteins, thus serving as a valuable tool for capturing cell surface interactions prior to cell lysis and further analysis such as immunoprecipitation. This feature is crucial in the study of membrane proteins, which are often integral to cell signaling and are popular targets for drug development. Given that membrane proteins frequently have roles in disease pathways, the ability to study them effectively using a crosslinker like DTSSP reinforces its importance in pharmacological research.
DTSSP also finds application in improving the specificity and reliability of bioconjugates in proteomics. Its specifications, including a purity standard of over 80% as confirmed by quantitative NMR, ensure consistent performance in complex biological systems. Each lot of DTSSP is manufactured to meet stringent quality controls, providing researchers with confidence in their experimental outputs. The ability to cleave the crosslinker post-reaction simplifies the analysis process, allowing for clearer interpretation of mass spectrometry data and enhancing the identification of novel protein interactions or modifications. Such precision is indispensable in identifying drug targets and understanding drug effects at the molecular level.
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BOC Sciences offers comprehensive services for ADC manufacturing, including antibody modification, linker chemistry, payload conjugation, and formulation development. In particular, our payload-linker customization service offers a convenient and fast raw material channel for many ADC researchers.
BOC Sciences provides one-stop site-specific conjugation services for amino acids, glycans, unnatural amino acids, and short peptide tags. In addition, cysteine conjugation, lysine conjugation, enzymatic conjugation, thio-engineered antibody can also be obtained quickly.
BOC Sciences offers a full range of linkers, including peptide linkers, PEG linkers, click chemistry, PROTAC linkers, non-cleavable linkers, etc. We also provide custom development services for chemically labile linkers and enzymatically cleavable linkers.